IMPLEMENTATION OF THE SYSTEM OF HONEY SAFETY CONTROL ON THE EXAMPLE OF ANALYZING OF RESIDUES OF TETRACYCLINE GROUP ANTIBIOTICS

The National Reference Laboratory of Drug Residues Control has implemented a system "classical" honey safety control based on the parameters antimicrobial drug residues: samples testing by screening enzyme-linked immunosorbent assay (ELISA) method and, in case positive or possibly occurring, – retesting confirmatory UPLC-MS/MS multi-method developed laboratory. article presents results ELISA validation for determination residual amounts tetracycline group antibiotics (tetracycline, oxytetracycline, chlortetracycline, doxycycline) using Tetracyclin® RIDASCREEN test kits R-Biopharm (Germany) and Oxytetracycline EuroProxima (the Netherlands) its verification means method.
 In accordance with Commission Implementing Regulation (EU) 2021/808, proposed manufacturers was carried out taking into account minimum required limit detection. fitness-for-purpose methods confirmed establishment main (technical threshold cut-off factor) (blank) fortified standard solutions at level ½ detection, according to "added-received" criterion. are simple carrying out, time- cost-effective, which their advantages.
 reliability obtained verified multi-method. comparative studies solution 2.5 5.0 μg/kg, as well interlaboratory accredited laboratory presented.